Hi,
I'm thinking about a whole genome resequencing experiment for the identification of selection signatures. I will perform a WGA because we have a very low DNA quantity. I have read that SNP calling is very affected, specially in those regions with an insufficient coverage. So, I wonder which would be an appropiate coverage for an amplified sample...maybe the double of that of the unamplified one?
Could anyone help me?
Thanks!
I'm thinking about a whole genome resequencing experiment for the identification of selection signatures. I will perform a WGA because we have a very low DNA quantity. I have read that SNP calling is very affected, specially in those regions with an insufficient coverage. So, I wonder which would be an appropiate coverage for an amplified sample...maybe the double of that of the unamplified one?
Could anyone help me?
Thanks!