Ligation without a Tails

[trainglot]

During library preparation I made a silly mistake.
After end repair I used buffer and enzyme for ligation skipping the A tailing.
Now the question: it’s still possible to recover the DNA?
Don’t hesitate to ask further informations

[nucacidhunter]

You can clean up reaction with a column or 1.8x bead and set up A tailing reaction if you did not add adapters. If you added adapters as well and fragment sizes are longer than 150 bp then clean up with 1.5x bead.