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I ran an RNA 6000 nano chip with RNA samples in all 12 wells, some randomly came back flagged with error: unexpected signal in fast region. Is there any way to troubleshoot this after the fact to get an accurate RIN number?
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You should be able to manually adjust the fast region signal allowance under the advanced settings and this will clear the error, allowing for a RIN to be called. -
Agilent tech support is really helpful with fixing these problems, if you are unsure how to do so. (Via phone or email them the run file)Comment
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