We have a set of data files coming from a multiplex sequencing run on HiScan SQ machine and now we need to obtain fastq file in txt format of our sample. Could someone indicates a process on commands line step by step to do this?This is the first experience with this kind of data.
Until now we are able to install OLB software and to launch the command as reported in the user-guide of Off-Line Basecaller v1.9 (nov.2010):
./bustard.py --CIF /srv/illumina/Runs/111006_H112_0131_AB0B0VABXX/Data/Intensities/ --make --with-qseq
At this point we don't know if the output directories containes the exact files in the exact format.
Thank's for your time and your help.
Until now we are able to install OLB software and to launch the command as reported in the user-guide of Off-Line Basecaller v1.9 (nov.2010):
./bustard.py --CIF /srv/illumina/Runs/111006_H112_0131_AB0B0VABXX/Data/Intensities/ --make --with-qseq
At this point we don't know if the output directories containes the exact files in the exact format.
Thank's for your time and your help.
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