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  • help about cufflinks for bacterial rna-seq data

    Hi,recently I am analysing bacterial rna-seq data from illumina GAII.Iwant to use cufflinks to caculate RPKM, I select the default options and get the result file as follow:
    tracking_id class_code nearest_ref_id gene_id gene_short_name tss_id locus length coverage FPKM FPKM_conf_lo FPKM_conf_hi FPKM_status
    That is all inthe final file. There is no values. I don't know why.
    So please help me if you know the reason. Is there any special options in cufflinks for analysing bacterial rna-seq data?
    Thank you very much!
    Best wishes!

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