I bet someone has asked this question before, but I'll ask it anyway. I have run Tophat and have the accepted hits bam file and the juntions.bed file. I'd like to know the number of reads in the bam file aligning to each junction coordinate in the bed file. I have tried to use bedtools with no success. Does anyone have any idea how to achieve this?. Essentially I'd like to know only the number of reads aligning to junctions.
Regards.
Regards.
Comment