Seqanswers Leaderboard Ad
Collapse
Announcement
Collapse
No announcement yet.
X
-
indeed. i tried this today and the difference in FPKM values between the two normalizations was constant out to 4 decimal places.
-
Oh I am very sorry! It is my problem: one set was processed slightly differently. :-(
Thanks, sdriscoll!
Leave a comment:
-
sounds fishy. i wanna try this out and see what results i get. I'll report back unless of course someone comes in an explains it.
Leave a comment:
-
cufflinks --compatible-hits-norm
Hi,
I am testing cufflinks with two different normalization methods: total-hits-norm and compatible-hits-norm. My understanding is that the only difference between these two methods is the FPKM denominator: one is the number of all hits, the other is the number of hits within the reference GTF. So the ratios of FPKM values calculated by two methods should be a single fixed number, which is the ratio of hits. But, the ratios I got is a distribution. Anybody have an idea of the exact meaning of compatible-hits-norm? Thanks!Tags: None
Latest Articles
Collapse
-
by seqadmin
The first FDA-approved CRISPR-based therapy marked the transition of therapeutic gene editing from a dream to reality1. CRISPR technologies have streamlined gene editing, and CRISPR screens have become an important approach for identifying genes involved in disease processes2. This technique introduces targeted mutations across numerous genes, enabling large-scale identification of gene functions, interactions, and pathways3. Identifying the full range...-
Channel: Articles
08-27-2024, 04:44 AM -
ad_right_rmr
Collapse
News
Collapse
Topics | Statistics | Last Post | ||
---|---|---|---|---|
Started by seqadmin, Yesterday, 08:02 AM
|
0 responses
10 views
0 likes
|
Last Post
by seqadmin
Yesterday, 08:02 AM
|
||
Started by seqadmin, 09-03-2024, 08:30 AM
|
0 responses
13 views
0 likes
|
Last Post
by seqadmin
09-03-2024, 08:30 AM
|
||
Started by seqadmin, 08-27-2024, 04:40 AM
|
0 responses
21 views
0 likes
|
Last Post
by seqadmin
08-27-2024, 04:40 AM
|
||
New Single-Molecule Sequencing Platform Introduces Advanced Features for High-Throughput Genomics
by seqadmin
Started by seqadmin, 08-22-2024, 05:00 AM
|
0 responses
358 views
0 likes
|
Last Post
by seqadmin
08-22-2024, 05:00 AM
|
Leave a comment: