==> Error: Splice sequence retrieval failed with err = -6

[Zimbobo]

Hello,

I ran tophat 2.0.0 and bowtie2-2.0.0-beta5 like this


bowtie2-build -f a.fa a
tophat -p 8 --phred64-quals --output-dir "." a reads.fa

The reads a single end.

tophat terminates with the message

Error: Splice sequence retrieval failed with err = -6


What am I doing wrong?

Thanks.