Tweet
I ran TopHat and the run was successful but I am unable to figure out why my junction.bed file is empty? can anybody guide?
thanx
thanx
-
Last edited by bharati; 06-05-2012, 03:54 AM. -
You'll need to provide a lot more information if you want an answer to that question. -
more information ?? like what?Comment
-
can u pls elaborateComment
-
Organism and platform used for sequencing. Did the Tophat run produce any errors? Do you have split reads in your alignment?Comment
-
actually i used the given command:
/home/tophat-2.0.0.Linux_x86_64/tophat -r -34 --mate-std-dev 36 /home/bowtie2-2.0.0-beta5/example/hg19/hg19 /data1/bowtie_aln/R1_RB002_zip_cat_trim_4_90_Q30_80_matched.fastq /data1/bowtie_aln/R2_RB002_zip_cat_trim_4_90_Q30_80_matched.fastqComment
-
do I need to specify some more options along with the above command? Did I missed some thing?Comment
-
Many organizations study rare diseases, but few have a mission as impactful as Rady Children’s Institute for Genomic Medicine (RCIGM). “We are all about changing outcomes for children,” explained Dr. Stephen Kingsmore, President and CEO of the group. The institute’s initial goal was to provide rapid diagnoses for critically ill children and shorten their diagnostic odyssey, a term used to describe the long and arduous process it takes patients to obtain an accurate...12-16-2024, 07:57 AM -
Innovations in next-generation sequencing technologies and techniques are driving more precise and comprehensive exploration of complex biological systems. Current advancements include improved accessibility for long-read sequencing and significant progress in single-cell and 3D genomics. This article explores some of the most impactful developments in the field over the past year.
Long-Read Sequencing
Long-read sequencing has seen remarkable advancements,...12-02-2024, 01:49 PM
Comment