Tweet
I have some RNA-seq data for statistic analysis. I do not have biological replicate for the data, so could anyone can give me some suggestion how to compare the significant expressed genes between two samples using Fisher's exact test in R? Thank you very much.
-
-
Fisher's test tests for independency between rows and columns in a table so I don't think you can use it. In fact, with only two samples there is not much that can be done (there has been a lot of discussion on SEQanswers about detecting DE genes from RNAseq in the absence of replication). You can look at the DESeq and edgeR packages from Bioconductor, which can estimate dispersion in absence of replication, but again, the results shouldn't be taken as conclusive.Originally posted by beefeng123 View Post
-
Many organizations study rare diseases, but few have a mission as impactful as Rady Children’s Institute for Genomic Medicine (RCIGM). “We are all about changing outcomes for children,” explained Dr. Stephen Kingsmore, President and CEO of the group. The institute’s initial goal was to provide rapid diagnoses for critically ill children and shorten their diagnostic odyssey, a term used to describe the long and arduous process it takes patients to obtain an accurate...12-16-2024, 07:57 AM -
Innovations in next-generation sequencing technologies and techniques are driving more precise and comprehensive exploration of complex biological systems. Current advancements include improved accessibility for long-read sequencing and significant progress in single-cell and 3D genomics. This article explores some of the most impactful developments in the field over the past year.
Long-Read Sequencing
Long-read sequencing has seen remarkable advancements,...12-02-2024, 01:49 PM
Comment