Hi everyone,
This is my first post, so please be gentle
I'm working on some Solexa data for a collaborator and have noticed that the quality (as determined by matches to the genome) of the sequence reads drop off very quickly beyond ~25nt.
Now that I have the actual Solexa read quality scores what kind of cut-offs to people use for throwing out 'junk' reads? Some informal discussions have suggested scores 30 and above..
Any thoughts?
Thanks,
Chris.
This is my first post, so please be gentle
I'm working on some Solexa data for a collaborator and have noticed that the quality (as determined by matches to the genome) of the sequence reads drop off very quickly beyond ~25nt.
Now that I have the actual Solexa read quality scores what kind of cut-offs to people use for throwing out 'junk' reads? Some informal discussions have suggested scores 30 and above..
Any thoughts?
Thanks,
Chris.
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