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I've been trimming paired reads with DynamicTrim based on phred scores and using LengthSort to separate out those under 15 bases. LengthSort separates them into 4 files: 2 for the good reads, one for those under 15 bases, and one for the mates of the reads that were under 15 bases (but which are themselves fine). Is there any way I can still use the reads who were unfortunate enough to have a <15 base mate, or are they complete junk since they no longer have a mate? I am currently using bwa for alignment
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Many organizations study rare diseases, but few have a mission as impactful as Rady Children’s Institute for Genomic Medicine (RCIGM). “We are all about changing outcomes for children,” explained Dr. Stephen Kingsmore, President and CEO of the group. The institute’s initial goal was to provide rapid diagnoses for critically ill children and shorten their diagnostic odyssey, a term used to describe the long and arduous process it takes patients to obtain an accurate...12-16-2024, 07:57 AM -
Innovations in next-generation sequencing technologies and techniques are driving more precise and comprehensive exploration of complex biological systems. Current advancements include improved accessibility for long-read sequencing and significant progress in single-cell and 3D genomics. This article explores some of the most impactful developments in the field over the past year.
Long-Read Sequencing
Long-read sequencing has seen remarkable advancements,...12-02-2024, 01:49 PM
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