I would like to display a histogram of mean mapped base phred quality (call quality, not mapping quality), with chromosomal location on the "x-axis"
I have recalibrated & realigned BAM files from Illumina sequencing for a small target.
Is it possible to extract this data presented by chromosome location?
Obviously, IGV will display individual reads, with individual base quality. Can the mean quality be automatically extracted?
Essentially, I would like to compare this with depth of coverage by chromosomal location, which coverageBed extracts from the BAM file directly.
Thanks.
I have recalibrated & realigned BAM files from Illumina sequencing for a small target.
Is it possible to extract this data presented by chromosome location?
Obviously, IGV will display individual reads, with individual base quality. Can the mean quality be automatically extracted?
Essentially, I would like to compare this with depth of coverage by chromosomal location, which coverageBed extracts from the BAM file directly.
Thanks.
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