Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • Extract mean mapped base phred quality by mapped location from BAM file

    I would like to display a histogram of mean mapped base phred quality (call quality, not mapping quality), with chromosomal location on the "x-axis"
    I have recalibrated & realigned BAM files from Illumina sequencing for a small target.
    Is it possible to extract this data presented by chromosome location?

    Obviously, IGV will display individual reads, with individual base quality. Can the mean quality be automatically extracted?

    Essentially, I would like to compare this with depth of coverage by chromosomal location, which coverageBed extracts from the BAM file directly.

    Thanks.

  • #2
    You could write a script that grabs the quality values from a BAM file and averages them over a sliding window.

    Comment


    • #3
      Thanks adaptivegenome.
      That is beyond my programming skills. I was hoping that someone may have done something similar already...
      I may make do with the BamUtil: stats Q20% as an alternative way of representing this data.
      The purpose is to try and identify the cause of incorrect variant calls... Is base-specific read quality a factor?

      Comment

      Latest Articles

      Collapse

      • seqadmin
        Advancing Precision Medicine for Rare Diseases in Children
        by seqadmin




        Many organizations study rare diseases, but few have a mission as impactful as Rady Children’s Institute for Genomic Medicine (RCIGM). “We are all about changing outcomes for children,” explained Dr. Stephen Kingsmore, President and CEO of the group. The institute’s initial goal was to provide rapid diagnoses for critically ill children and shorten their diagnostic odyssey, a term used to describe the long and arduous process it takes patients to obtain an accurate...
        12-16-2024, 07:57 AM
      • seqadmin
        Recent Advances in Sequencing Technologies
        by seqadmin



        Innovations in next-generation sequencing technologies and techniques are driving more precise and comprehensive exploration of complex biological systems. Current advancements include improved accessibility for long-read sequencing and significant progress in single-cell and 3D genomics. This article explores some of the most impactful developments in the field over the past year.

        Long-Read Sequencing
        Long-read sequencing has seen remarkable advancements,...
        12-02-2024, 01:49 PM

      ad_right_rmr

      Collapse

      News

      Collapse

      Topics Statistics Last Post
      Started by seqadmin, 12-17-2024, 10:28 AM
      0 responses
      26 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 12-13-2024, 08:24 AM
      0 responses
      42 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 12-12-2024, 07:41 AM
      0 responses
      28 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 12-11-2024, 07:45 AM
      0 responses
      42 views
      0 likes
      Last Post seqadmin  
      Working...
      X