Hello all,
I use Tophat to align the RNAseq reads to human genome. However, I want to visualize the coverage across the regions of interest.
I know that the IGV can show the wiggles (or bigwig) file.
So, can anyone tell me how to make the wiggle file from the tophat bam file ?
Thanks so much.
I use Tophat to align the RNAseq reads to human genome. However, I want to visualize the coverage across the regions of interest.
I know that the IGV can show the wiggles (or bigwig) file.
So, can anyone tell me how to make the wiggle file from the tophat bam file ?
Thanks so much.
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