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  • a_mt
    Member
    • Jul 2012
    • 34

    splitting longer reads into two smaller reads for bowtie input

    Hi all,

    I have sequence data from Illumina GA with read length of 95 bp's (after trimming).
    Now I am using a tool which works with bowtie. Since my read length is above 50bp's and bowtie doesn't allow gapped alignment, I want to split the read into two shorter reads.

    Is there any tool to do this??

    Thank you.

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