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splitting longer reads into two smaller reads for bowtie input
Hi all,
I have sequence data from Illumina GA with read length of 95 bp's (after trimming).
Now I am using a tool which works with bowtie. Since my read length is above 50bp's and bowtie doesn't allow gapped alignment, I want to split the read into two shorter reads.
Is there any tool to do this??
Thank you.
I have sequence data from Illumina GA with read length of 95 bp's (after trimming).
Now I am using a tool which works with bowtie. Since my read length is above 50bp's and bowtie doesn't allow gapped alignment, I want to split the read into two shorter reads.
Is there any tool to do this??
Thank you.