Hi,
I have an interesting situation. I am trying to perform a DE analysis on miRNAseq data using mirDeep2 from the miRNAs_expressed_all_samples_now.csv file.
I am interested in the DE of my mature miRNA sequences however, there are sevreal cases where my mature miRNA has a different precursor sequence, for example:
mmu-let-7a-5p 54439.00 mmu-let-7a-1 54439.00 54439.00 5414.39
mmu-let-7a-5p 54422.00 mmu-let-7a-2 54422.00 54422.00 5412.70
The expression values are similar but not the same. Has anyone dealt with this before and could you give me some advice? I am confused on whether i should (i) treat these mature miRNAs with different precursors as unique expression values (ii), if should use expression values from just one of these miRNAs or (iii), if i should add the expression values from both entires of mmu-let-7a-5p to get the actual expression value.
Thanks in advance for any assistance with this query.
I have an interesting situation. I am trying to perform a DE analysis on miRNAseq data using mirDeep2 from the miRNAs_expressed_all_samples_now.csv file.
I am interested in the DE of my mature miRNA sequences however, there are sevreal cases where my mature miRNA has a different precursor sequence, for example:
mmu-let-7a-5p 54439.00 mmu-let-7a-1 54439.00 54439.00 5414.39
mmu-let-7a-5p 54422.00 mmu-let-7a-2 54422.00 54422.00 5412.70
The expression values are similar but not the same. Has anyone dealt with this before and could you give me some advice? I am confused on whether i should (i) treat these mature miRNAs with different precursors as unique expression values (ii), if should use expression values from just one of these miRNAs or (iii), if i should add the expression values from both entires of mmu-let-7a-5p to get the actual expression value.
Thanks in advance for any assistance with this query.
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