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Dear users,
I'm running a file fastq of microRNA reads with bfast, using as reference the microRNA precursor sequence.
The step "localalign" runs only if I set the parameter -u (Do ungapped local alignment).
I run the bfast postprocess step as:
>bfast postprocess -f hsa_human_miRNAprec_miRBaseV13.fasta -i sample_622T_9.baf -a 3 -O 3 -o sample
but it returns me an error and it crashes:
In function "GetReverseComplimentAnyCaseBase": Fatal Error[OutOfRange]. Message: Could not understand sequence base.
Has someone had the same problems?
Thanks very much!
Bye bye,
Maria Elena
I'm running a file fastq of microRNA reads with bfast, using as reference the microRNA precursor sequence.
The step "localalign" runs only if I set the parameter -u (Do ungapped local alignment).
I run the bfast postprocess step as:
>bfast postprocess -f hsa_human_miRNAprec_miRBaseV13.fasta -i sample_622T_9.baf -a 3 -O 3 -o sample
but it returns me an error and it crashes:
In function "GetReverseComplimentAnyCaseBase": Fatal Error[OutOfRange]. Message: Could not understand sequence base.
Has someone had the same problems?
Thanks very much!
Bye bye,
Maria Elena