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kit.

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Join Date: Feb 2013

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hy PCR is included in library preparation for NGS?

04-16-2013, 10:16 PM

One important step for analyzing whole exome sequencing is to mark duplication so as to decrease miscalling of SNPs.

It may be a silly question, but I am just wondering why PCR is included in library preparation? If this step is skipped, then there's no need to remove duplicates?
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