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  • SOAP can't align read

    HI everyone. I'm new here. I got a problem blocked me for days and plz help me.
    I want to use SOAP to align my paired-end reads. command:

    ./SOAP -a /home/d-l/L008_R1_001.fastq -b /home/d-l/L008_R2_001.fastq -D /home/d-l/ref.fa.index -o /home/d-l/pairmapped.txt -2 /home/d-l/singlemapped.txt -u /home/d-l/unmapped.txt -m 500 -x 10000

    But pairmapped.txt & singlemapped.txt are all size 0. unmapped.txt is large. No read mapped! Then I tried single read:

    ./SOAP -a /home/d-l/L008_R1_001.fastq -D /home/d-l/ref.fa.index -o /home/d-l/singletest.txt

    But still singletest.txt is size 0.

    I am going to be crazy. I can't explain to my supervisor. Any one who have succeed?

  • #2
    Well... is there a reason you must use SOAP? Have you tried different programs like BWA?

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    • #3
      Originally posted by oiiio View Post
      Well... is there a reason you must use SOAP? Have you tried different programs like BWA?
      Not must. Actually I want to use mapView to call SNPs(someone told me that it can). mapView only accept SOAP output for pair-end read.

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