Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • Cuffdiff problem output!!!

    i have some problem related to cuffidiff output, i used this command for replicates:

    cuffdiff -o diff_out -b <genome.fa> -p 10 -L CT,SS --total-hits-norm -u cuffmerge_experiment/merged.gtf tophat_exp/exp1/accepted_hits.bam,tophat_exp1/exp2/accepted_hits.bam tophat_exp/exp3/accepted_hits.bam,tophat_exp/exp4/accepted_hits.bam

    in my output file i found gene column is empty:

    TCONS_00000001 XLOC_000001 - C11044824:403-1007 CT SS OK 4.25634 4.05292 -0.0706522 0.148488 0.881958 0.999985 no
    TCONS_00000002 XLOC_000002 - C11047824:1-1042 CT SS OK 4.96333 5.54938 0.161018 -0.40853 0.682884 0.999985 no
    TCONS_00000003 XLOC_000003 - C11048020:0-588 CT SS OK 3.66925 4.25115 0.212368 -0.422853 0.672403 0.999985 no
    TCONS_00000004 XLOC_000004 - C11048338:216-588 CT SS OK 0.795992 0.604691 -0.396557 0.258201 0.796252 0.999985 no
    TCONS_00000005 XLOC_000005 - C11049822:426-1035 CT SS OK 0.359691 0.790309 1.13566 -0.675737 0.499208 0.999985 no

    i cant understand how can be its possible, if any one have suggestion please guide me.

    upto cuffmerge i found gene in my file like this:

    [CODE]C11111420 Cufflinks exon 726 1304 . - . gene_id "XLOC_000033"; transcript_id "TCONS_00000034"; exon_number "1"; oId "Ca_28192"; nearest_ref "Ca_28192"; class_code "="; tss_id "TSS33"; p_id "P11";
    C11112120 Cufflinks exon 391 1218 . + . gene_id "XLOC_000034"; transcript_id "TCONS_00000035"; exon_number "1"; oId "Ca_27742"; nearest_ref "Ca_27742"; class_code "="; tss_id "TSS34"; p_id "P12";
    C11112120 Cufflinks exon 1577 1657 . + . gene_id "XLOC_000034"; transcript_id "TCONS_00000035"; exon_number "2"; oId "Ca_27742"; nearest_ref "Ca_27742"; class_code "="; tss_id "TSS34"; p_id "P12";
    C11112120 Cufflinks exon 1929 2126 . - . gene_id "XLOC_000035"; transcript_id "TCONS_00000036"; exon_number "1"; oId "Ca_27743"; nearest_ref "Ca_27743"; class_code "="; tss_id "TSS35"; p_id "P13";
    C11115550 Cufflinks exon 6 533 . + . gene_id "XLOC_000036"; transcript_id "TCONS_00000037"; exon_number "1"; oId "Ca_27676"; nearest_ref "Ca_27676"; class_code "="; tss_id "TSS36"; p_id "P14";
    C11115550 Cufflinks exon 2561 2800 . - . gene_id "XLOC_000037"; transcript_id "TCONS_00000038"; exon_number "1"; oId "Ca_27677"; nearest_ref "Ca_2767
    Last edited by ALOK N PATEL; 11-27-2013, 03:49 AM.

  • #2
    cross posted on http://www.biostars.org/p/87526

    Comment


    • #3
      This problem is being faced by my friend he posted it on biostar but he was not able to do the same in seqanswers!!

      Comment

      Latest Articles

      Collapse

      • seqadmin
        Advancing Precision Medicine for Rare Diseases in Children
        by seqadmin




        Many organizations study rare diseases, but few have a mission as impactful as Rady Children’s Institute for Genomic Medicine (RCIGM). “We are all about changing outcomes for children,” explained Dr. Stephen Kingsmore, President and CEO of the group. The institute’s initial goal was to provide rapid diagnoses for critically ill children and shorten their diagnostic odyssey, a term used to describe the long and arduous process it takes patients to obtain an accurate...
        12-16-2024, 07:57 AM
      • seqadmin
        Recent Advances in Sequencing Technologies
        by seqadmin



        Innovations in next-generation sequencing technologies and techniques are driving more precise and comprehensive exploration of complex biological systems. Current advancements include improved accessibility for long-read sequencing and significant progress in single-cell and 3D genomics. This article explores some of the most impactful developments in the field over the past year.

        Long-Read Sequencing
        Long-read sequencing has seen remarkable advancements,...
        12-02-2024, 01:49 PM

      ad_right_rmr

      Collapse

      News

      Collapse

      Topics Statistics Last Post
      Started by seqadmin, 12-17-2024, 10:28 AM
      0 responses
      23 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 12-13-2024, 08:24 AM
      0 responses
      42 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 12-12-2024, 07:41 AM
      0 responses
      28 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 12-11-2024, 07:45 AM
      0 responses
      42 views
      0 likes
      Last Post seqadmin  
      Working...
      X