Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • Looking for replicated conditions

    Hello! I'm a newbie and I'm trying to write a function which can read how much replicated conditions there are in an experimental design in a given case. For example, if I have a design (for example an input like a data.frame) like the following:

    sample condition
    1 C1.sam A
    2 C2.sam A
    3 C2.sam A
    4 Infect1.sam B
    5 Infect2.sam B
    6 Infect3.sam B

    How can I access the number of replicates? (perhaps some function similar to apply?) Cause obviously there are three replicates called "A" and three replicates called "B", but I don´t know how to store this in a variable, so I can work with that information later on the script.

    Thanks and sorry for my beginner's English

  • #2
    It sort of depends on how you want to use things later on. If the dataframe is called "d", then:

    Code:
    table(d)
    That will give you a table with the conditions as names and counts as values. In general, though, you can just "~d$condition" or use model.matrix.

    Comment


    • #3
      Thank u dpryan. The table-function worked =)

      What if I wanted to use a function like that with my counts, not with my design table? A data frame like this for example:

      Control1 Control2 Control3 Infect1 Infect2 Infect3
      4 34 23 12 2 33
      6 14 15 17 32 22

      I want to get the information that the condition "control" is replicated 3 times, and condition "infect" is replicated 3 times, too (so that R get just the information in common ignoring the numbers).

      Comment


      • #4
        There's no simple function for that since R would have to already know your general labeling format. Presuming that numbers at the end of sample names can always be stripped, then the general idea would be to:

        1) Save the names in another variable (foo <- names(d))
        2) Remove numbers from the end (normally this would be a regex)
        3) You could then use things like "table" or whatever on the results.

        Of course, if you new ahead of time what the groups were you could just count how many contain a phrase (i.e., use grep), but I'm assuming you want to do this as part of a general purpose pipeline.

        Comment

        Latest Articles

        Collapse

        • seqadmin
          Advancing Precision Medicine for Rare Diseases in Children
          by seqadmin




          Many organizations study rare diseases, but few have a mission as impactful as Rady Children’s Institute for Genomic Medicine (RCIGM). “We are all about changing outcomes for children,” explained Dr. Stephen Kingsmore, President and CEO of the group. The institute’s initial goal was to provide rapid diagnoses for critically ill children and shorten their diagnostic odyssey, a term used to describe the long and arduous process it takes patients to obtain an accurate...
          12-16-2024, 07:57 AM
        • seqadmin
          Recent Advances in Sequencing Technologies
          by seqadmin



          Innovations in next-generation sequencing technologies and techniques are driving more precise and comprehensive exploration of complex biological systems. Current advancements include improved accessibility for long-read sequencing and significant progress in single-cell and 3D genomics. This article explores some of the most impactful developments in the field over the past year.

          Long-Read Sequencing
          Long-read sequencing has seen remarkable advancements,...
          12-02-2024, 01:49 PM

        ad_right_rmr

        Collapse

        News

        Collapse

        Topics Statistics Last Post
        Started by seqadmin, 12-17-2024, 10:28 AM
        0 responses
        22 views
        0 likes
        Last Post seqadmin  
        Started by seqadmin, 12-13-2024, 08:24 AM
        0 responses
        42 views
        0 likes
        Last Post seqadmin  
        Started by seqadmin, 12-12-2024, 07:41 AM
        0 responses
        28 views
        0 likes
        Last Post seqadmin  
        Started by seqadmin, 12-11-2024, 07:45 AM
        0 responses
        42 views
        0 likes
        Last Post seqadmin  
        Working...
        X