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I am extremely new to Biopython and was hoping someone could help me. I have downloaded the complete GenBank files for Salmonella enterica serovar Typhimurium SL1344 (it has 3 plasmids and one chromosome and I have concatenated all of them into one file). I have a list of 123 gene names that I would like to make a Multi-FASTA file for. Is there an easy way to do this? Thank you so much for any and all help.
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I suggest you read http://www2.warwick.ac.uk/fac/sci/mo...genbank2fasta/ but also why not download the matching per-gene FASTA file from the NCBI and filter that?
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Like all molecular biology applications, next-generation sequencing (NGS) workflows require diligent quality control (QC) measures to ensure accurate and reproducible results. Proper QC begins at nucleic acid extraction and continues all the way through to data analysis. This article outlines the key QC steps in an NGS workflow, along with the commonly used tools and techniques.
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