I have a list of sequences (cDNA) that I want to find in many different organisms. I have obtained genomic scaffolds for these organisms from various locations.
I BLAST my cDNA sequences against the genomic scaffolds and find the best reciprocal BLAST hits. The problem is that the hits are broken up (presumably by introns in the genomic scaffolds). Is there an efficient method or tool for extracting just coding sequences from a genomic scaffold?
thanks.
I BLAST my cDNA sequences against the genomic scaffolds and find the best reciprocal BLAST hits. The problem is that the hits are broken up (presumably by introns in the genomic scaffolds). Is there an efficient method or tool for extracting just coding sequences from a genomic scaffold?
thanks.
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