Hello all, i would really appreciate some help with the situation i find myself in.
I am trying to do snp calling and visualisation for some genomes i have assembled.
I would love to be able to use ginger/parsnp to visualise snps and connect them back to genes but for this i need an annotated genome of the day zero strain we put into people (and then sampled, collecting bugs every few weeks)
My supervisor has forbidden me from adding the day zero genome to public databases. So i need a well annotated strain of my bacteria (none are available for this particular strain)
I tried RAST and the genbank files it generated didn't work with harvest or mauve or IGV or BRIG.
I am trying PROKKA but in order to get a good result i need to use the --centre "xxx" command and specify a sequencing centre ID.
Do any of you know what the sequencing ID for sanger is?
Or do any of you have any idea of another way i can annotate my sequence
Much obliged
Thank you
I am trying to do snp calling and visualisation for some genomes i have assembled.
I would love to be able to use ginger/parsnp to visualise snps and connect them back to genes but for this i need an annotated genome of the day zero strain we put into people (and then sampled, collecting bugs every few weeks)
My supervisor has forbidden me from adding the day zero genome to public databases. So i need a well annotated strain of my bacteria (none are available for this particular strain)
I tried RAST and the genbank files it generated didn't work with harvest or mauve or IGV or BRIG.
I am trying PROKKA but in order to get a good result i need to use the --centre "xxx" command and specify a sequencing centre ID.
Do any of you know what the sequencing ID for sanger is?
Or do any of you have any idea of another way i can annotate my sequence
Much obliged
Thank you
Comment