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Hi everyone,
I have a draft genome which was binned from a bacterial metagenome. I know for certain that the genome doesn't have a 16s gene in it, but should be fairly complete with 42 single copy bacterial genes present. I wish to identify the taxonomy of this genome. By BLASTing several ORFs in the genome against NCBI's nr database I have a reasonable idea to which taxon it belongs, but when running MAUVE to align my binned genome and a draft genome from NCBI I don't get a nicely re-ordered genome, only a mess...
so my question has two parts:
1. Is there an online tool to which I can upload my draft genome and it will run it again its database to produce similarity values to known genomes ?
2. Which other tools would you recommend in order to asses the completeness of the genome ?
Thanks!
I have a draft genome which was binned from a bacterial metagenome. I know for certain that the genome doesn't have a 16s gene in it, but should be fairly complete with 42 single copy bacterial genes present. I wish to identify the taxonomy of this genome. By BLASTing several ORFs in the genome against NCBI's nr database I have a reasonable idea to which taxon it belongs, but when running MAUVE to align my binned genome and a draft genome from NCBI I don't get a nicely re-ordered genome, only a mess...
so my question has two parts:
1. Is there an online tool to which I can upload my draft genome and it will run it again its database to produce similarity values to known genomes ?
2. Which other tools would you recommend in order to asses the completeness of the genome ?
Thanks!