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How to get genome wide statistics with MISO
I've done work with specific genes using MISO and obtaining various isoform/exon-centric outputs and visualizing with sashimi plot. All that has been great, but now I want to do some broader genome-wide analyses.
For example, out of the 20,000 something genes in my summary file, how many are differentially expressed between various stages of the cell (d20 vs d30, etc)? How would I answer that? I'm obviously not going to go through the comparison file and count each gene that become differentially expressed, so how do I make miso do something like that?
For example, out of the 20,000 something genes in my summary file, how many are differentially expressed between various stages of the cell (d20 vs d30, etc)? How would I answer that? I'm obviously not going to go through the comparison file and count each gene that become differentially expressed, so how do I make miso do something like that?