Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • Can you spot whats wrong with this .gff3?

    Hello,
    I've been working with tophat for a while pretty successfully, but for some reason this one data set will not cooperate. Using the .gff file I downloaded for the corresponding genome I get:

    [Fri Jul 9 15:45:42 2010] Reading known junctions from GFF file
    Warning: TopHat did not find any junctions in GFF file
    I've tried making several adjustments to the file to make it fit the prescribed format. I've also double checked that the first column matches whats in the bowtie index using bowtie-inspect. Maybe someone else has had a similar experience? I've tried several variants on the following format and nothing helps, which makes me thing is probably something I've overlooked and I am out of ideas.

    Chr01 glean gene 47206323 47208329 . - . ID=BGIOSGA000006;Name=BGIOSGA000006;
    Chr01 glean mRNA 47206323 47208329 0.999993 - . ID=BGIOSGA000006;Name=BGIOSGA000006-TA;Parent=BGIOSGA000006;ID=BGIOSGA000006.50;Name=BGIOSGA000006-TA;ID=BGIOSGA000006;
    Chr01 glean exon 47208026 47208329 . - 0 Parent=BGIOSGA000006;ID=BGIOSGA000006.51;
    Chr01 glean exon 47207590 47207951 . - 2 Parent=BGIOSGA000006;ID=BGIOSGA000006.52;
    Chr01 glean exon 47207158 47207394 . - 0 Parent=BGIOSGA000006;ID=BGIOSGA000006.53;
    Chr01 glean exon 47206323 47206556 . - 0 Parent=BGIOSGA000006;ID=BGIOSGA000006.54;
    Thanks in advance.

Latest Articles

Collapse

  • seqadmin
    Recent Innovations in Spatial Biology
    by seqadmin


    Spatial biology is an exciting field that encompasses a wide range of techniques and technologies aimed at mapping the organization and interactions of various biomolecules in their native environments. As this area of research progresses, new tools and methodologies are being introduced, accompanied by efforts to establish benchmarking standards and drive technological innovation.

    3D Genomics
    While spatial biology often involves studying proteins and RNAs in their...
    01-01-2025, 07:30 PM
  • seqadmin
    Advancing Precision Medicine for Rare Diseases in Children
    by seqadmin




    Many organizations study rare diseases, but few have a mission as impactful as Rady Children’s Institute for Genomic Medicine (RCIGM). “We are all about changing outcomes for children,” explained Dr. Stephen Kingsmore, President and CEO of the group. The institute’s initial goal was to provide rapid diagnoses for critically ill children and shorten their diagnostic odyssey, a term used to describe the long and arduous process it takes patients to obtain an accurate...
    12-16-2024, 07:57 AM

ad_right_rmr

Collapse

News

Collapse

Topics Statistics Last Post
Started by seqadmin, 01-09-2025, 04:04 PM
0 responses
432 views
0 likes
Last Post seqadmin  
Started by seqadmin, 01-09-2025, 09:42 AM
0 responses
441 views
0 likes
Last Post seqadmin  
Started by seqadmin, 01-08-2025, 03:17 PM
0 responses
453 views
0 likes
Last Post seqadmin  
Started by seqadmin, 01-03-2025, 11:18 AM
1 response
50 views
1 like
Last Post Tonia
by Tonia
 
Working...
X