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  • how to set seed length in Tophat

    how to set seed length? When i try to run Tophat, it always showed "seed length: 90bp", but my reads are only 90bp, i would like to set the seed length to 35bp, but i fail to find any parameter in Manual.

  • #2
    Under Advanced options:

    --segment-length Each read is cut up into segments, each at least this long. These segments are mapped independently. The default is 25.

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    • #3
      Originally posted by nicklin View Post
      Under Advanced options:

      --segment-length Each read is cut up into segments, each at least this long. These segments are mapped independently. The default is 25.
      this option does not change the seed length. I did not set this option, but when i try to run Tophat, it still showed seed length was 90bp.

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