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  • xinwu
    Member
    • Jul 2010
    • 33

    #1

    Where can I find public dataset of NGS?

    Hi all,
    Where can I download public dataset of NGS? I only know one:
    • NCBI SRA(Short Reads Archive)

    Data for whole genome resequencing is prefered.
    Thanks.
    Last edited by xinwu; 09-01-2010, 12:38 AM.
  • krobison
    Senior Member
    • Nov 2007
    • 734

    #2
    Given that SRA has a huge number of datasets, what is it you can't find there?

    I believe the European Nucleotide Archive freely exchanges with SRA, but with a time lag, so they are nearly identical in content.

    Best way to query SRA I've found is via BioConductor module SRAdb -- it truly runs rings around the current web interfaces (where European archive is better than NCBI by a small notch), assuming you know SQL (which is easy enough to learn if you don't).

    It also depends on what you are planning to do with the dataset; there are a limited number of actual assemblies available in BAM format which can be accessed using SAMtools via FTP/HTTP; see this prior thread for more on that.

    Comment

    • xinwu
      Member
      • Jul 2010
      • 33

      #3
      Hi Krobison,
      Thanks a lot. We're planning to make a shorts reads assembly software review and comparison and look for a de facto standard data set as our benchmark. Although human genome is a well candidate, we prefer to find some plant genome. Thanks for your tips.

      Comment

      • Joann
        Senior Member
        • Oct 2008
        • 230

        #4
        Any suggestions

        Hi Keith,

        Can you suggest a good standard list of SRA's for general reference use and international benchmarking purposes? You know, a variety of samples--a plant, an animal, a microorganism or even an environmental sample. And details on where these data sets might be obtained by a user via the web.

        Comment

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