Hello, I am using "stats.sh" command in BBtools to check my assembled metagenomes. I am looking for N50 and the largest contig. In my case, there is a line shows "Main genome contig N/L50: 7688/759" and "Max contig length: 5.905 KB". I know N50 and L50? What is N/L50 here? Does this mean N50 = 7688 and L50=759? Why my max contig length is shorter than N50? Is this possbile? (details see attached files)
A C G T N IUPAC Other GC GC_stdev
0.2126 0.2873 0.2874 0.2127 0.0000 0.0000 0.0000 0.5747 0.0976
Main genome scaffold total: 19074
Main genome contig total: 19074
Main genome scaffold sequence total: 15.193 MB
Main genome contig sequence total: 15.193 MB 0.000% gap
Main genome scaffold N/L50: 7688/759
Main genome contig N/L50: 7688/759
Main genome scaffold N/L90: 16532/636
Main genome contig N/L90: 16532/636
Max scaffold length: 5.905 KB
Max contig length: 5.905 KB
Number of scaffolds > 50 KB: 0
% main genome in scaffolds > 50 KB: 0.00%
A C G T N IUPAC Other GC GC_stdev
0.2126 0.2873 0.2874 0.2127 0.0000 0.0000 0.0000 0.5747 0.0976
Main genome scaffold total: 19074
Main genome contig total: 19074
Main genome scaffold sequence total: 15.193 MB
Main genome contig sequence total: 15.193 MB 0.000% gap
Main genome scaffold N/L50: 7688/759
Main genome contig N/L50: 7688/759
Main genome scaffold N/L90: 16532/636
Main genome contig N/L90: 16532/636
Max scaffold length: 5.905 KB
Max contig length: 5.905 KB
Number of scaffolds > 50 KB: 0
% main genome in scaffolds > 50 KB: 0.00%
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