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Hi, golharam and simonandrews
Thanks! I am using WinXP. I will try to run SeqMonk on my desktop first to see if it works.
-c
Thanks! I am using WinXP. I will try to run SeqMonk on my desktop first to see if it works.
-c
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This week I discovered that using BigWig files for coverage data works really nicely in GBrowse.
I would really love to have a simple plotting tool to take per chromosome coverage data from something like a BigWig file and plot a nice looking coverage figure. I'm thinking of coding it myself in (probably) python or (maybe) R but if anyone can point me to examples to get started that would be great. Although some genome browsers make coverage plots, they just don't look nice for publication use IMHO.Last edited by natstreet; 10-01-2010, 08:10 AM.Comment
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Hi, simonandrews
I just noticed that the SeqMonk Release Notes talks about SeqMonk v0.12.0 but it seems I can only find SeqMonk v0.11.0 online. Do you know where to find v0.12.0?
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I can see v0.11.0 listed at their website and if you swap 11 to 12 in the download URL there is something there to be downloaded
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Sorry, I'd failed to copy over the new version of the download page from our staging server. If you try again now the links should be in place (if you don't see them press shift+refresh in your browser to force a cache update).Originally posted by cliff View Post
Simon.Comment
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IGV scripting function
Use IGV to output plots of a region of a bam file with the following sample script
attached is an example (note path to bam and snapshotDirectory need to be absolute not relative)
new
load myfile.bam
snapshotDirectory mySnapshotDirectory
genome hg18
goto chr1:65,289,335-65,309,335
sort position
collapse
snapshotComment
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<shameless plug>Originally posted by arnoldliao View PostUse IGV to output plots of a region of a bam file with the following sample script
attached is an example (note path to bam and snapshotDirectory need to be absolute not relative)
new
load myfile.bam
snapshotDirectory mySnapshotDirectory
genome hg18
goto chr1:65,289,335-65,309,335
sort position
collapse
snapshot
Note that BEDTools has a tool called bedToIGV that will automatically create an IGV batch script from a BED, GFF, or VCF file.
</shameless plug>Comment
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The human gut contains trillions of microorganisms that impact digestion, immune functions, and overall health1. Despite major breakthroughs, we’re only beginning to understand the full extent of the microbiome’s influence on health and disease. Advances in next-generation sequencing and spatial biology have opened new windows into this complex environment, yet many questions remain. This article highlights two recent studies exploring how diet influences microbial...02-24-2025, 06:31 AM -
Like all molecular biology applications, next-generation sequencing (NGS) workflows require diligent quality control (QC) measures to ensure accurate and reproducible results. Proper QC begins at nucleic acid extraction and continues all the way through to data analysis. This article outlines the key QC steps in an NGS workflow, along with the commonly used tools and techniques.
Nucleic Acid Quality Control
Preparing for NGS starts with isolating the...02-10-2025, 01:58 PM
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