Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • Comparing two VCF files with vcftools, chromosome out ouf order error message

    Hi,

    I'm using the vcftools for the first time. I'm trying to compare two VCF files with the following command line:

    Code:
    vcftools --vcf Sample_All.vcf --diff Sample_indA.vcf --diff-site --out Multiple_vs_indA
    and I get the following error message:

    Code:
    Error: Both files must be sorted in the same chromosomal order.
    chr1 in file 2 appears to be out of order.
    But I know that in both VCF file I have the chr1 included, so, why do I have this message?
    Plus I have tried to had the
    Code:
    --not-chr chr1
    parameter to my command line, as I understand it was advised to do in this case, but then I have the same error message but on chr2 ...
    Is it normal? What should I do to resolve this issue? Or maybe it is just a warning message?

    Thank you
    C.

  • #2
    Hello cmonat,

    take a look inside both vcf files and check whether they are ordered correct by chromosom and position.

    fin swimmer

    Comment

    Latest Articles

    Collapse

    • seqadmin
      Advancing Precision Medicine for Rare Diseases in Children
      by seqadmin




      Many organizations study rare diseases, but few have a mission as impactful as Rady Children’s Institute for Genomic Medicine (RCIGM). “We are all about changing outcomes for children,” explained Dr. Stephen Kingsmore, President and CEO of the group. The institute’s initial goal was to provide rapid diagnoses for critically ill children and shorten their diagnostic odyssey, a term used to describe the long and arduous process it takes patients to obtain an accurate...
      12-16-2024, 07:57 AM
    • seqadmin
      Recent Advances in Sequencing Technologies
      by seqadmin



      Innovations in next-generation sequencing technologies and techniques are driving more precise and comprehensive exploration of complex biological systems. Current advancements include improved accessibility for long-read sequencing and significant progress in single-cell and 3D genomics. This article explores some of the most impactful developments in the field over the past year.

      Long-Read Sequencing
      Long-read sequencing has seen remarkable advancements,...
      12-02-2024, 01:49 PM

    ad_right_rmr

    Collapse

    News

    Collapse

    Topics Statistics Last Post
    Started by seqadmin, 12-17-2024, 10:28 AM
    0 responses
    33 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 12-13-2024, 08:24 AM
    0 responses
    49 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 12-12-2024, 07:41 AM
    0 responses
    34 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 12-11-2024, 07:45 AM
    0 responses
    46 views
    0 likes
    Last Post seqadmin  
    Working...
    X