Hi everyone, I was wondering if anyone knows exactly how does GATK HaplotypeCaller treat unmapped reads.
The thing is, first GATK defines <<active regions>> in which it will operate, after this, it goes one by one and builds an assembly graph using the reference sequence. Once it has the graph, it "takes each read in turn and attempts to match it to a segment of the graph; whenever portions....".
The question is, when it takes EACH READ in turn, it means, the reads that mapped there, right? not every single read in the bam file.
Is there anyone who knows the excplicit answer?
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The first FDA-approved CRISPR-based therapy marked the transition of therapeutic gene editing from a dream to reality1. CRISPR technologies have streamlined gene editing, and CRISPR screens have become an important approach for identifying genes involved in disease processes2. This technique introduces targeted mutations across numerous genes, enabling large-scale identification of gene functions, interactions, and pathways3. Identifying the full range...08-27-2024, 04:44 AM