Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • Combining multiple Piccard commands with Bash

    I'm new to using bash for piping. I'm currently trying to combine (pipe) multiple Piccard commands with Bash.
    I have two executions that both work when I run them one by one in a bash script:

    **********************************************

    Command 1
    #!/bin/bash

    # Set paths
    picardtools='PATH/picard.jar'
    bams="PATH/BAM"
    outpath="PATH/out"

    for i in Bam1 Bam2
    do
    java -jar $picardtools SortSam INPUT=$bams/"$i" OUTPUT=$outpath/"$i" SORT_ORDER=coordinate CREATE_INDEX=TRUE VALIDATION_STRINGENCY=LENIENT
    done

    Command 2
    #!/bin/bash

    # Set paths
    picardtools='PATH/picard.jar'
    bams="PATH/BAM"
    outpath="PATH/out"
    outpath2="PATH/out2"

    for i in Bam1 Bam2
    do
    java -jar $picardtools AddOrReplaceReadGroups INPUT=$outpath/"$i" OUTPUT=$outpath2/"$i" SORT_ORDER=coordinate RGID="$i" RGLB="$i" RGPL=illumina RGPU="$i" RGSM="$i" CREATE_INDEX=True
    done

    **********************************************

    I have now tried to pipe these commands, but it no longer works. I have tried 2 ways of doing the piping:


    Option 1:
    #!/bin/bash

    # Set paths
    picardtools='PATH/picard.jar'
    bams="PATH/BAM"
    outpath="PATH/out"
    outpath2="PATH/out2"

    for i in Bam1 Bam2
    do
    java -jar $picardtools SortSam INPUT=$bams/"$i" OUTPUT=/dev/stdout SORT_ORDER=coordinate CREATE_INDEX=TRUE VALIDATION_STRINGENCY=LENIENT | \
    java -jar $picardtools AddOrReplaceReadGroups INPUT=/dev/stdin OUTPUT=$outpath2/"$i" SORT_ORDER=coordinate RGID="$i" RGLB="$i" RGPL=illumina RGPU="$i" RGSM="$i" CREATE_INDEX=True
    done


    Option 2:
    #!/bin/bash

    # Set paths
    picardtools='PATH/picard.jar'
    bams="PATH/BAM"
    outpath="PATH/out"
    outpath2="PATH/out2"

    for i in Bam1 Bam2
    do
    java -jar $picardtools SortSam INPUT=$bams/"$i" OUTPUT=$outpath/"$i" SORT_ORDER=coordinate CREATE_INDEX=TRUE VALIDATION_STRINGENCY=LENIENT
    java -jar $picardtools AddOrReplaceReadGroups INPUT=$outpath/"$i" OUTPUT=$outpath2/"$i" SORT_ORDER=coordinate RGID="$i" RGLB="$i" RGPL=illumina RGPU="$i" RGSM="$i" CREATE_INDEX=True
    done


    Neither of these two options work. Can someone help me finding the bug?

Latest Articles

Collapse

  • seqadmin
    Advancing Precision Medicine for Rare Diseases in Children
    by seqadmin




    Many organizations study rare diseases, but few have a mission as impactful as Rady Children’s Institute for Genomic Medicine (RCIGM). “We are all about changing outcomes for children,” explained Dr. Stephen Kingsmore, President and CEO of the group. The institute’s initial goal was to provide rapid diagnoses for critically ill children and shorten their diagnostic odyssey, a term used to describe the long and arduous process it takes patients to obtain an accurate...
    12-16-2024, 07:57 AM
  • seqadmin
    Recent Advances in Sequencing Technologies
    by seqadmin



    Innovations in next-generation sequencing technologies and techniques are driving more precise and comprehensive exploration of complex biological systems. Current advancements include improved accessibility for long-read sequencing and significant progress in single-cell and 3D genomics. This article explores some of the most impactful developments in the field over the past year.

    Long-Read Sequencing
    Long-read sequencing has seen remarkable advancements,...
    12-02-2024, 01:49 PM

ad_right_rmr

Collapse

News

Collapse

Topics Statistics Last Post
Started by seqadmin, 12-17-2024, 10:28 AM
0 responses
26 views
0 likes
Last Post seqadmin  
Started by seqadmin, 12-13-2024, 08:24 AM
0 responses
42 views
0 likes
Last Post seqadmin  
Started by seqadmin, 12-12-2024, 07:41 AM
0 responses
28 views
0 likes
Last Post seqadmin  
Started by seqadmin, 12-11-2024, 07:45 AM
0 responses
42 views
0 likes
Last Post seqadmin  
Working...
X