Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • Wrong blast results

    I'm sorry if this is a naive question, but I get an odd thing going on. I'm performing blast remotely in this way:
    blastn -task megablast -outfmt 6 std ssciname -reward 1 -penalty -3 -db nt_v5 -max_target_seqs 100 -word_size 14 -query input_file + " -taxidlist " + Plants_DB -out blastfile

    However, when I see my remote results, I get a species whose seqid gives me Vaccinium oxycoccos. And when I put this seqid in blast online, the species is totally different.

    Can someone help me fix this problem?
    Thanks

Latest Articles

Collapse

  • seqadmin
    Investigating the Gut Microbiome Through Diet and Spatial Biology
    by seqadmin




    The human gut contains trillions of microorganisms that impact digestion, immune functions, and overall health1. Despite major breakthroughs, we’re only beginning to understand the full extent of the microbiome’s influence on health and disease. Advances in next-generation sequencing and spatial biology have opened new windows into this complex environment, yet many questions remain. This article highlights two recent studies exploring how diet influences microbial...
    02-24-2025, 06:31 AM
  • seqadmin
    Quality Control Essentials for Next-Generation Sequencing Workflows
    by seqadmin




    Like all molecular biology applications, next-generation sequencing (NGS) workflows require diligent quality control (QC) measures to ensure accurate and reproducible results. Proper QC begins at nucleic acid extraction and continues all the way through to data analysis. This article outlines the key QC steps in an NGS workflow, along with the commonly used tools and techniques.

    Nucleic Acid Quality Control
    Preparing for NGS starts with isolating the...
    02-10-2025, 01:58 PM

ad_right_rmr

Collapse

News

Collapse

Topics Statistics Last Post
Started by seqadmin, 03-03-2025, 01:15 PM
0 responses
154 views
0 likes
Last Post seqadmin  
Started by seqadmin, 02-28-2025, 12:58 PM
0 responses
238 views
0 likes
Last Post seqadmin  
Started by seqadmin, 02-24-2025, 02:48 PM
0 responses
608 views
0 likes
Last Post seqadmin  
Started by seqadmin, 02-21-2025, 02:46 PM
0 responses
263 views
0 likes
Last Post seqadmin  
Working...
X