Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • coverage depth, bedtools and RNA-Seq experiment

    I want to estimate the % of a transcriptome covered by an RNA-Seq experiment.

    I tried to use BEDTools: one file consisted of exons only coordinates (from UCSC), the other file consisted of TopHat alignments.

    The results show that 70% of the transcriptome has coverage depth 0.

    I was expecting a much smaller percentage, given that I started with 100,000,000 reads x 2 (PE) and the total length of the transcriptome is 17,470,227.

    Am I making a big mistake here?

    ps. when I say "transcriptome", I mean the collection of all exons.

  • #2
    Can you show us the command you used to generate that value?

    Comment


    • #3
      Originally posted by natstreet View Post
      Can you show us the command you used to generate that value?
      coverageBed -abam my_accepted_hits.bam -b rn4_UCSC_kg_exons.bed -hist -s -split

      Comment

      Latest Articles

      Collapse

      • seqadmin
        Investigating the Gut Microbiome Through Diet and Spatial Biology
        by seqadmin




        The human gut contains trillions of microorganisms that impact digestion, immune functions, and overall health1. Despite major breakthroughs, we’re only beginning to understand the full extent of the microbiome’s influence on health and disease. Advances in next-generation sequencing and spatial biology have opened new windows into this complex environment, yet many questions remain. This article highlights two recent studies exploring how diet influences microbial...
        02-24-2025, 06:31 AM
      • seqadmin
        Quality Control Essentials for Next-Generation Sequencing Workflows
        by seqadmin




        Like all molecular biology applications, next-generation sequencing (NGS) workflows require diligent quality control (QC) measures to ensure accurate and reproducible results. Proper QC begins at nucleic acid extraction and continues all the way through to data analysis. This article outlines the key QC steps in an NGS workflow, along with the commonly used tools and techniques.

        Nucleic Acid Quality Control
        Preparing for NGS starts with isolating the...
        02-10-2025, 01:58 PM

      ad_right_rmr

      Collapse

      News

      Collapse

      Topics Statistics Last Post
      Started by seqadmin, 03-03-2025, 01:15 PM
      0 responses
      154 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 02-28-2025, 12:58 PM
      0 responses
      238 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 02-24-2025, 02:48 PM
      0 responses
      607 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 02-21-2025, 02:46 PM
      0 responses
      263 views
      0 likes
      Last Post seqadmin  
      Working...
      X