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Anybody have this problem before? A brand new box of SV oil; one tube missing...
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Nope, but I've had leaky oil tubes and cups before... -
missing reagents
Haven't seen missing oil tubes....yet. But we did have this XLR70 kit the other day with no ATP. The tube wasn't cracked or leaking.....it was just empty!Comment
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We have a XL+ kit without DTT tube!!!!!!!!
I write to sequencing service and he replace immediately the kit.
...no comment!
do you check all the kits when you receive them?Comment
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We had a sleeve of FLX+ reagents (50mL tubes) where 2 of the reagents were in the wrong positions...
They replaced the kit without issue.Comment
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Interesting.
Yeah, I've started checking everything as soon as it shows up. Didn't think it would be necessary, but after seeing all of these replies I think differently.Comment
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two tubes in wrong position!!!!!
I will check the kits better now.
Do you have te lot number of this kit?Comment
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Double checking is a good idea - we're all human and mistakes happen. We struggled to find a list of reagents and their proper positions in any of the 454 manuals/reagent inserts which was a bit alarming. I understand they will include something like this in their next update to the manuals. Until then, make your own list
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Thought I would contribute - nothing dramatic but; my supervisor couldn't figure out why the sipper manifold wouldn't lower completely when he was doing a maintenance wash until he discovered.........Comment
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We had the following:
1) Polymerase at half the level required
2) Empty Apyrase Tupe
3) Cracked XL sequencing tray tubes (leaking)Comment
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Many organizations study rare diseases, but few have a mission as impactful as Rady Children’s Institute for Genomic Medicine (RCIGM). “We are all about changing outcomes for children,” explained Dr. Stephen Kingsmore, President and CEO of the group. The institute’s initial goal was to provide rapid diagnoses for critically ill children and shorten their diagnostic odyssey, a term used to describe the long and arduous process it takes patients to obtain an accurate...12-16-2024, 07:57 AM -
Innovations in next-generation sequencing technologies and techniques are driving more precise and comprehensive exploration of complex biological systems. Current advancements include improved accessibility for long-read sequencing and significant progress in single-cell and 3D genomics. This article explores some of the most impactful developments in the field over the past year.
Long-Read Sequencing
Long-read sequencing has seen remarkable advancements,...12-02-2024, 01:49 PM
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