Hello Seqers,
We have a illumina technician installing our HiSeq2000 as I write and I am trying to make some final improvements on my library constructions especially in the area of quality control.
I saw that life tech has ERCC spike in controls pretty well incorporated into their protocols and pipeline. When I was looking for this in combination with illumina protocols I found nothing. When I called illumina tech support no one had ever heard of ERCC.
Does anyone have experience with the ERCC controls?
Do they really add that much more information in terms of helping helping me interpret my data?
Why does illumina not use them/support them/know about them?
Thanks a lot for your insights,
O.
We have a illumina technician installing our HiSeq2000 as I write and I am trying to make some final improvements on my library constructions especially in the area of quality control.
I saw that life tech has ERCC spike in controls pretty well incorporated into their protocols and pipeline. When I was looking for this in combination with illumina protocols I found nothing. When I called illumina tech support no one had ever heard of ERCC.
Does anyone have experience with the ERCC controls?
Do they really add that much more information in terms of helping helping me interpret my data?
Why does illumina not use them/support them/know about them?
Thanks a lot for your insights,
O.
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