I am looking to run some libraries I initially prepped for HiSeq via Trueseq RNA V2 on the MiSeq and have a question.
So I have read that the protocols are compatible, but the MiSeq only does PE reads and because of this your adapters need to be complimentary?
I am not sure exactly what this means? And where in the library prep I would have to make note of this? Or is all the PE SE stuff determined later in the clustering and the Single Index libraries I prepped should be good to go for the MiSeq because I will cluster on the MiSeq so all should be well?
So I have read that the protocols are compatible, but the MiSeq only does PE reads and because of this your adapters need to be complimentary?
I am not sure exactly what this means? And where in the library prep I would have to make note of this? Or is all the PE SE stuff determined later in the clustering and the Single Index libraries I prepped should be good to go for the MiSeq because I will cluster on the MiSeq so all should be well?
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