Priming Flongle Flowcell

[d_araneda_r]

Hi!
I hope you are very well, what protocols are there for priming the Flongle Flowcells?
Thanks.

[gringer]

Well, there's the official ONT protocol which requires special tips and very careful standard plunge pipetting in order to not damage pores, and there's the negative-pressure gentle loading protocol which is more similar to the SpotON port loading method (search for "gentle loading") on the ONT community.

[d_araneda_r]

Well, there's the official ONT protocol which requires special tips and very careful standard plunge pipetting in order to not damage pores, and there's the negative-pressure gentle loading protocol which is more similar to the SpotON port loading method (search for "gentle loading") on the ONT community.

it was very useful, thank you very much

[helendam]

Hello!
Regarding your question, Flongle flow cells are compatible with both R9.4 and R10.3 chemistry, and the protocol for priming them depends on the specific chemistry you are using.
For R9.4 chemistry, the Flongle flow cells can be primed using the protocol for priming MinION flow cells, which involves flushing the flow cell with running buffer and then washing with loading buffer. The detailed protocol can be found in the MinION flow cell user guide, available on the Oxford Nanopore Technologies website.
For R10.3 chemistry, the priming protocol is slightly different and involves priming the flow cell with EP buffer and then washing with loading buffer. The detailed protocol for priming R10.3 Flongle flow cells can also be found on the Oxford Nanopore Technologies website.
It is important to carefully follow the priming protocols for your specific chemistry to ensure optimal performance and to prevent damage to the flow cell.backrooms game

[gringer]

As far as I'm aware, Flongle flow cells have never been available for R9.4 nor R10.3. R9.4 was an earlier pore technology that stopped being used by ONT around 2019. R10.3 I'm less sure about. It might have been an earlier R10 technology, but was never offered on Flongle (except possibly to a sprinkling of labs for early-access testing).

Flongle flow cells are currently available on the ONT store in R9.4.1 and R10.4.1 pore formats ($810 USD for 12 flow cells).