unnatural bases recognition

[yaximik]

Hi,

I know that PacBio analysis pipeline can distinguish modified bases by analyzing incorporation kinetics, such as 5mC. Does anybody know about deamination products, like dU? Some polymerases just cannot get over it. In general I am interested whether this methodology can be applied to analysis of ancient DNA, obviously if long enough molecules have survived. Crazy idea, but still...

[rhall]

Sounds like a great experiment. Other forms of DNA damage have already been shown to be detectable with PacBio:
http://www.genomeintegrity.com/content/2/1/10