Hi,
I'm working with 16S data, and have been using PEAR (http://bioinformatics.oxfordjournals...cs.btt593.full) for merging my reads (thanks PEAR people, great tool!). This has been going great but my most recent set of data has lots of unassembled reads (> 50%) - can I include these into a 16S analysis pipeline and if so, how? Every reference I've read says PE data must be merged before 16S analysis (QIIME, mothur and UPARSE).
thanks.
I'm working with 16S data, and have been using PEAR (http://bioinformatics.oxfordjournals...cs.btt593.full) for merging my reads (thanks PEAR people, great tool!). This has been going great but my most recent set of data has lots of unassembled reads (> 50%) - can I include these into a 16S analysis pipeline and if so, how? Every reference I've read says PE data must be merged before 16S analysis (QIIME, mothur and UPARSE).
thanks.
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