An inside look at Pacific Biosciences technologies...

[ECO]

The New York Times has a good article on the current technology in use at Pacific Biosciences in Menlo Park.

http://www.nytimes.com/2008/02/09/business/09genome.html?_r=1&oref=slogin&pagewanted=print

S.M.R.T. sequencing (Single Molecule Real Time) Sounds like 454 on steroids. Instead of a "picotiter plate" and pyrophosphate detection, these guys are using a "zero mode waveguide" and phospho-linked fluors.

All I can think of is that old Simpson's episode...

[sci_guy]

Amazing stuff! The technology backgrounder is a good document for getting up to speed with SMRT. http://www.pacificbiosciences.com/as...ckgrounder.pdf

A press release to GenomeWeb today reveals that the machine will be available (if one is optimistic) in 2010. http://www.genomeweb.com/issues/news/144976-1.html

[ECO]

Here is a link to one of their very recent publications...

http://www.pnas.org/cgi/content/abstract/105/4/1176

[deepak_bala]

Here's a link to their animation explaining the process.

http://www.pacificbiosciences.com/video_lg.html

[NextGenSeq]

Thanks for the "unbiased" promotion of PacBio.

(Note: It might be a good idea to put a disclaimer when you post that you work for them similar to the press releases from the marketing team of companies anticipating to go public.)

[ECO]

Thanks for the "unbiased" promotion of PacBio.

(Note: It might be a good idea to put a disclaimer when you post that you work for them similar to the press releases from the marketing team of companies anticipating to go public.)

Hey there...I assume you're talking to me. This disclaimer has been there since the day I started at PB, and this thread was posted almost a year before I joined.

Not sure why you keep bringing it up...I've been as open as possible since day one, and if you'd checked the date on this thread before posting what I feel is a pretty snide comment, you'd know that.

As I said the last time you brought this up, if you would like to start a dialog about my day-job employer and how that may or may not affect the unbiased nature of the site...please do so in the Site Feedback forum and I'll answer any question and consider all opinions.