Does anyone have any experience as to what happens when AMPURE XP reagent goes bad? We have been using it routinely to purify high molecular weight DNA for Nanopore sequencing but also of a sudden, we're getting zero DNA recovery. This is associated with expensive clumping of the beads after incubation with the DNA, and a failure of the bead clumps to dissociate during "elution."
Im looking for information on how "expired" beads normally behave: do they fail to bind DNA in the first place, or do they do what we're seeing: hold on to it so tightly that it's impossible to release. I don't need suggestions of things to try to release the DNA - we've tried EVERYTHING. Just needing to know how to tell if beads have gone bad - one of our bottles is only 8 months old.
Im looking for information on how "expired" beads normally behave: do they fail to bind DNA in the first place, or do they do what we're seeing: hold on to it so tightly that it's impossible to release. I don't need suggestions of things to try to release the DNA - we've tried EVERYTHING. Just needing to know how to tell if beads have gone bad - one of our bottles is only 8 months old.
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