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Old 08-09-2016, 02:16 AM   #1
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Default Different alignment rate of Tophat with and without GTF file


I am new to RNA seq analysis. I am working on non-model organism. I have Genome and de-novo-assembled transcriptome. Using PASA I mapped the transcriptome to genome and obtained GTF file.

When I mapped my RNAseq reads to Genome using tophat by providing GTF file obtained from PASA, I get overall alignment rate of 91.4% while when ran Tophat without GTF option, I got overall alignment rate of 78%.

Why is the difference. Kindly guide me
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Old 08-09-2016, 03:24 AM   #2
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GenoMax is offline   Reply With Quote

rna sequencing, tophat

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