I hope somebody can help me out here.
I often get double band in ChIP-seq lib prep for Illumina platform. One peak is about 200bp, and additional peak around 260bp. This happens often, but not all the time, really annoying. Does any body have clues?
Could it be bad RNAase or Proteinase K on treating the ChIP samples? Because a recent exp has one old sample working fine (single 200bp peak), but not the new sample.
Or too much adaptor in the library prep?
Thanks in advance for your help!
Jh
I often get double band in ChIP-seq lib prep for Illumina platform. One peak is about 200bp, and additional peak around 260bp. This happens often, but not all the time, really annoying. Does any body have clues?
Could it be bad RNAase or Proteinase K on treating the ChIP samples? Because a recent exp has one old sample working fine (single 200bp peak), but not the new sample.
Or too much adaptor in the library prep?
Thanks in advance for your help!
Jh
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