Hi, all. I am a freshman using illumina machines.
We have a Hiseq2500 and did PE125 runs. But the results to me are curious.
Please see the images uploaded. The base distribution plots are splited at the very beginning of read 1 and the late cycles of read 2(technical saying read 3). Are these causing by the library themselves or our operation faults or bad reagent lots? How to solve these?
We have a Hiseq2500 and did PE125 runs. But the results to me are curious.
Please see the images uploaded. The base distribution plots are splited at the very beginning of read 1 and the late cycles of read 2(technical saying read 3). Are these causing by the library themselves or our operation faults or bad reagent lots? How to solve these?
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