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  • Test for overlap of R1 and R2

    I have 300 bp paired-end reads from an Illumina MiSeq. Technically, if the reads are full-length, I should have about 60-70 bp overlap between them.

    Can someone suggest a quick way for me to determine what the overlap is between each set of paired reads and output that metric in some sort of useful form (i.e. a graph)? I just want to be able to do a quick QC on the data before I hand it over for the full informatics pipeline.

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    Discussion of next-gen sequencing related bioinformatics: resources, algorithms, open source efforts, etc

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