We tried for the first time using DNA (amplicons) previously purified with AMPure XP and then emPCR at 0.25 cpb with the GS FLX kits with the GS Junior emPCR kits. Our enrichment went from 15% to 40-50% (ran duplicates). Has anyone else used the GS Junior emPCR kits and do you have any recommendations. We are going to try again using .1 cpb.
Also any feedback on quality of library from amplicons using AMPure? We are getting a high percentage of dot+mixed as well as short. Roche thinks it may be the library source.
Also any feedback on quality of library from amplicons using AMPure? We are getting a high percentage of dot+mixed as well as short. Roche thinks it may be the library source.
Comment