Hi,
I was wondering if anyone has problems with space characters in the header of a fastq file
We have two technical replicates and out sequencing people told us it will save us time, if we merge the two files and analyse them together.
The problem is that the header are the same with the exception of the number of the technical replicate:
Q1: Are space character allowed in the header of the fastq file?
When we analysed the merged file we got the same results as the first original file of the two.
This led me to the thought, that such spaces and everything that followed are being ignored. We are working with maqgene to try and find SNPs in C. elegans.
Q2: Does it make sense at all to merge the two technical replicates to save analysis time? Do I loose information by merging them?
Thanks for the help
Assa
I was wondering if anyone has problems with space characters in the header of a fastq file
We have two technical replicates and out sequencing people told us it will save us time, if we merge the two files and analyse them together.
The problem is that the header are the same with the exception of the number of the technical replicate:
Code:
technical replicate 1 @HWI-ST0764:77:C03HCACXX:4:1101:1242:2075 1:Y:0:TTAGGC technical replicate 2 @HWI-ST0764:77:C03HCACXX:4:1101:1242:2075 2:Y:0:TTAGGC
When we analysed the merged file we got the same results as the first original file of the two.
This led me to the thought, that such spaces and everything that followed are being ignored. We are working with maqgene to try and find SNPs in C. elegans.
Q2: Does it make sense at all to merge the two technical replicates to save analysis time? Do I loose information by merging them?
Thanks for the help
Assa
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